![]() All tests were conducted on the same cohorts of animals. The MS cohort originally consisted of 9 MS and 8 MC mice while the MSEW cohort was comprised of 9 MSEW mice and 12 MC mice. During the course of testing, one cage in the MC group in the MSEW cohort exhibited excessive fighting and three mice progressively sustained severe injuries and were excluded from some tests. Other exclusions are listed in the figure legends. In the maternal control (MC) group, with the exception of routine bi-weekly cage changes, pups were left untouched with the dams until weaning at P21. For both maternal separation procedures, dams were moved from home cages to a clean cage with ad libitum food and water during separation. At the end of the separation period, both pups and dams were returned to the home cage.Īdditionally, cages containing the dams were placed in a separate room to prevent any potential communication among the dams and pups. ![]() In the maternal separation group (MS), pups were removed from their home cages from P2 to P14 for 3 hours daily at randomized time points during the light cycle. From P2 to 7, pups were placed together on a heating pad maintained at 37℃ during the separation period. ![]() From P7 to 14, pups were placed onto separate containers individually without heating. ![]()
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